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알림판

 
작성일 : 20-03-04 13:15
RNA 합성 시작
 글쓴이 : biostem
조회 : 171  
 BioStem RNA Synthesis
1.siRNA and single strand RNA
Project description Unit Purification  Price (\) TAT Remark
Conventional siRNA(15~25bp) 2 OD HPLC 117,000 Around 7days  
5 OD HPLC 190,000  
10 OD HPLC 337,000  
Note : Conventional siRNA provides a annealed double strand with two overhangs dT at the 3' end by double Strand
2、siRNA package
Project description specifications Price (\) Remark
siRNA oligo for the target gene 3 sites 2 OD HPLC 514,000 Around 7day
Negative control siRNA oligo 1 pair 1 OD HPLC Around 7day
FAM labeled negative control siRNA oligo 1 pair 1 OD HPLC Around 7day
RNAi positive control(GAPDH) siRNA oligo 1 pair 1 OD HPLC Around 7day
3、miRNA
Project  description Specifications/Price (\) Remark
5nmole 10nmole 20nmole >20nmole
miRNA Mimics                                  (Double chain annealing product) 117,000 176,000 176,000 inquiry Around 7day
miRNA Mimics nagetive control 29,000 58,000 73,000 inquiry Around 7day
miRNA inhibitor(RNA strands) 117,000 176,000 308,000 inquiry Around 7day
miRNA inhibitor nagetive control 29,000 58,000 73,000 inquiry Around 7day
Note:    (1) All products were purified by HPLC and passed strict quality control by Mass.
            (2) All RNA is produced in the clean workshop to strictly control endotoxin                                                                                                                                                      and meet the requirements of cell level and animal level.
          (3) Partial modified and long-stranded RNAs are not listed and shall be inquired.
4、shRNA
Project  description Unit Price (\) TAT Remark
shRNA Plasmid expression vector (pGPU6) 50μg 264,000 2 week  
shRNA Plasmid expression vector  (pGPH1) 50μg 264,000 2 week  
ShRNA Plasmid expression vector (pGPU6/Neo) 50μg 264,000 2 week  
ShRNA Plasmid expression vector(pGPH1/Neo) 50μg 264,000 2 week  
ShRNA Plasmid expression vector (pGPU6/Hygro) 50μg 264,000 2 week  
ShRNA Plasmid expression vector (pGPH1/Hygro) 50μg 264,000 2 week  
ShRNA Plasmid expression vector (pGPU6/GFP/Neo) 50μg 264,000 2 week  
ShRNA Plasmid expression vector (pGPH1/GFP/Neo) 50μg 264,000 2 week  
ShRNA Plasmid expression vector(pGPU6/RFP/Neo) 50μg 264,000 2 week  
ShRNA Plasmid expression vector (pGPH1/RFP/Neo ) 50μg 264,000 2 week  
5、Note
(1) 1OD=2.5nmole/ 20uM Stock Solution: Put 125ul DEPC Water Per 1OD(20uM=20pmole/ul) 
(2) Overhang dtdt increases siRNA stability and binding efficency with target mRNA(2'-Ome or 2'-Fluoro same effect)
(3) If you want candidate siRNA design, let us know Accession# orGene ID(Free)
(4) Must provide mature Id for miRNA mimics or Inhibitor Order(Example:has-miR-21)